An archaeal protein evolutionarily conserved in prokaryotes is a zinc-dependent metalloprotease

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An archaeal protein evolutionarily conserved in prokaryotes is a zinc-dependent metalloprotease

A putative protease gene (tldD) was previously identified from studying tolerance of letD encoding the CcdB toxin of a toxin-antidote system of the F plasmid in Escherichia coli. While this gene is evolutionarily conserved in archaea and bacteria, the proteolytic activity of encoded proteins remained to be demonstrated experimentally. Here we studied Sso0660, an archaeal TldD homologue encoded ...

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Archaeal DNA - alkyltransferase 
 
 1 Activity and Regulation of an Archaeal DNA - Alkyltransferase : a Conserved Protein Involved

Background:
DNA-alkyltransferases repair mutagenic and carcinogenic alkylation DNA lesions. Results:
A thermophilic archaeal DNAalkyltransferase is degraded after alkylation in vivo. A novel assay is applied to study its activity in vitro. Conclusion:
The archaeal DNA-alkyltransferase shows structure, activity and in vivo regulation similar to its human homolog. Significance:
The function and r...

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ژورنال

عنوان ژورنال: Bioscience Reports

سال: 2012

ISSN: 0144-8463,1573-4935

DOI: 10.1042/bsr20120074